Journal: Clinical and Developmental Immunology

Article Title: Automated Indirect Immunofluorescence Evaluation of Antinuclear Autoantibodies on HEp-2 Cells

doi: 10.1155/2012/651058

Figure Lengend Snippet: EUROPattern Microscope for automated acquisition of high-resolution immunofluorescence images. (a) Motorized microscope including camera, controlled LED, microscope control device, optional eyepieces, (b) matrix code reader, and (c) slide magazine. The microscope stage and the magazine are surrounded by a casing keeping out the sunlight and protecting the substrate fluorescence from fading. The microscope is part of the EUROPattern Suite, which additionally contains a laboratory management software (EUROLabOffice) and an automatic pattern recognition software (EUROPattern).

Article Snippet: The EUROPattern Suite (Euroimmun) consists of an automated EUROPattern Microscope, the laboratory management software EUROLabOffice, and the pattern recognition software EUROPattern.

Techniques: Microscopy, Immunofluorescence, Control, Fluorescence, Software

Journal: Clinical and Developmental Immunology

Article Title: Automated Indirect Immunofluorescence Evaluation of Antinuclear Autoantibodies on HEp-2 Cells

doi: 10.1155/2012/651058

Figure Lengend Snippet: Graphical user interface of EUROPattern. For each patient sample, EUROPattern displays the images of different sample dilutions, preliminary or additional results, and the software-generated result (recognized pattern, antibody titer, and calculated confidence value) on one report form. The software-generated result can be confirmed by mouse click or, if necessary, modified and specified using a readily displayed list of fluorescence patterns.

Article Snippet: The EUROPattern Suite (Euroimmun) consists of an automated EUROPattern Microscope, the laboratory management software EUROLabOffice, and the pattern recognition software EUROPattern.

Techniques: Software, Generated, Modification, Fluorescence

Journal: Clinical and Developmental Immunology

Article Title: Automated Indirect Immunofluorescence Evaluation of Antinuclear Autoantibodies on HEp-2 Cells

doi: 10.1155/2012/651058

Figure Lengend Snippet: Comparison of software-based and visual positive/negative classification.

Article Snippet: The EUROPattern Suite (Euroimmun) consists of an automated EUROPattern Microscope, the laboratory management software EUROLabOffice, and the pattern recognition software EUROPattern.

Techniques: Comparison, Software

Journal: Clinical and Developmental Immunology

Article Title: Automated Indirect Immunofluorescence Evaluation of Antinuclear Autoantibodies on HEp-2 Cells

doi: 10.1155/2012/651058

Figure Lengend Snippet: Main pattern recognition by EUROPattern.

Article Snippet: The EUROPattern Suite (Euroimmun) consists of an automated EUROPattern Microscope, the laboratory management software EUROLabOffice, and the pattern recognition software EUROPattern.

Techniques:

Journal: Nature immunology

Article Title: TGF-β is responsible for NK cell immaturity during ontogeny and increased susceptibility to infection during mouse infancy

doi: 10.1038/ni.2388

Figure Lengend Snippet: ( a – d ) CD11c dnR and wild type mice were injected ( i.p. ) twice a day with BrdU for 3 days. On day 3, mice were sacrificed and frequency of cycling cells was determined in the bone marrow. ( a – b ) FACS plots show the distribution of BrdU staining among total mNK cells ( a ) and gated mNK cells at stages D, E, and F ( b ). ( c ) Graph shows the frequency of cycling cells in pNK, iNK, and mNK cells from CD11c dnR (black circle) versus wild type (white circle) mice. ( d ) Graph shows the frequency of cycling cells in mNK cells at stages D, E, and F from CD11c dnR (black circle) versus wild type (white circle) mice. Data in a , d are representative of three independent experiments with n = 2 mice per experiment and results in c , d show all 6 individual mice. ( e – f ) mNK cells at stages D, E, and F were sorted from the bone marrow. mRNA was isolated and cDNA was subjected to pathway-specific qPCR for analysis of cell cycle genes ( e ) or SYBR Green qPCR for analysis of transcription factors T-bet, GATA-3, and IRF-2 ( f ). Data were analyzed using Global Pattern Recognition analytical software ( e ) or 2-33C3 method ( f ) and results were expressed as fold of change in CD11c dnR versus wild type samples. Data in e , f are representative of three independent cell sorting with samples pooled from n = 12 CD11c dnR and 25 WT mice.

Article Snippet: For cell cycle genes, we used a customized cell cycle qPCR array according to the manufacturer’s instructions (Lonza), and data were analyzed using Global Pattern Recognition analytical software (Lonza).

Techniques: Injection, BrdU Staining, Isolation, SYBR Green Assay, Software, FACS

Journal: Psychopharmacology

Article Title: Myocardial infarction sensitizes medial prefrontal cortex to inhibitory effect of locus coeruleus stimulation in rats

doi: 10.1007/s00213-016-4305-y

Figure Lengend Snippet: Example of ECG waveforms collected during LC stimulation in a rat subjected to CAL (a). Three sequential ECG waveforms are shown in each row. Sequential wave forms are shown before start of stimulation (Pre-stim), during stimulation and after stimulation (Post-stim). Note that arrhythmias unique from the prestimulus baseline rhythms develop midway through stimulation and did not revert immediately upon cessation of LC stimulation. bVertical plots indicating percent bigeminy, preventricular contraction (PVC), and ventricular tachycardic (VT) rhythms detected during real or mock LC stimulation in rats subjected to CAL and sham ligation. Symbols represent percent of total waveforms (beats) detected during real or mock stimulation that were of the specified waveform subtype and distinct from any waveform detected during the prestimulus time period for each individual animal. Horizontal bar indicates group mean, and vertical bar is SEM; **P < 0.01

Article Snippet: The ECG pattern recognition software ECG-Auto (EMKA Technologies, Falls Church, VA) was used to detect arrhythmias.

Techniques: Ligation